van Galen et al. introduce Mint-ChIP, an approach for multiplexed ChIP-seq on small cell numbers. The approach allows quantitative comparisons of global and locus-specific histone modification levels. The technology is demonstrated by mapping hematopoietic stem cell chromatin landscapes and quantifying changes in leukemia cells treated with epigenetic inhibitors. Detailed protocol now available on the Resources page.
Highlights
– Mint-ChIP enables highly multiplexed ChIP-seq on low-input samples
– Quantitative precision is achieved by normalizing histone modifications to total H3
– Active and repressed regions are mapped in human hematopoietic stem cells
A Multiplexed System for Quantitative Comparisons of Chromatin Landscapes
van Galen, Peter et al. Molecular Cell.