Single-Cell RNA-Seq Reveals AML Hierarchies Relevant to Disease Progression and Immunity.
van Galen P, Hovestadt V, Wadsworth Ii MH, Hughes TK, Griffin GK, Battaglia S, Verga JA, Stephansky J, Pastika TJ, Lombardi Story J, Pinkus GS, Pozdnyakova O, Galinsky I, Stone RM, Graubert TA, Shalek AK, Aster JC, Lane AA, Bernstein BE.
Cell. 2019 Mar 7, Epub 2019 Feb 28.
Highlights
- Single-cell RNA-seq reveals diverse malignant, stromal, and immune cells
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Malignant cells vary in cell cycle, hypoxia, and epithelial expression programs
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A partial EMT program (p-EMT) at leading edge is regulated by the microenvironment
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Computational modeling refines TCGA subtypes, linking p-EMT to metastasis